| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 1 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rabbit | IgG | Polyclonal | Abcam | ab185703 | Unconjugated | NA | Stock | Multiplexed 2D Imaging | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 2 |
| Q8TDQ0 | Primary Antibody | Tim-3 | Human | Rat | IgG2a | 344823 | R&D Systems | MAB2365 | Unconjugated | AB_2232900 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0003-4379-8967 | NA | 0000-0003-4379-8967 | 3 |
Publications
Additional Notes
- Evaluated in human lymph node with T cell markers (CD3 and PD-1). Antibody labels antigen experienced T cells and myeloid cells based on morphology and location.
- Evaluated in human lymph node with T cell markers (CD3 and PD-1). Antibody does not label expected cell types.
- Evaluated in human tonsil FFPE samples with T cell markers (CD3 and PD-1). Antibody does not label antigen experienced T cells and myeloid cells. No signal in tonsil.