| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-1431-1398 [1, 2] | NA | 0000-0003-1431-1398 | 1 | |
| P30548 | Primary Antibody | NK1.1 | Mouse | Mouse | IgG2a | PK136 | BioLegend | 108731 | BV421 | AB_10895916 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [2, 3] | NA | 0000-0003-4379-8967 |
Publications
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A. Gola et al., “Commensal-driven immune zonation of the liver promotes host defence”, Nature, 589(7840):131-136, 2021, doi:10.1038/s41586-020-2977-2.
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A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
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A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.
A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.
Additional Notes
- For additional details on how to prepare samples (perfusion, no detergent) see [Gola et al. 2020].