UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Small Intestine NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
P24788 Primary Antibody CD11b Mouse Rat IgG2b 5C6 Bio-Rad MCA711F FITC AB_1100617 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 30 minutes Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)