| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| O97799 | Primary Antibody | CD117 | Canine | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific | BS-0672R | Unconjugated | NA | Stock | Multiplexed 2D Imaging | FFPE | Skin | Melanoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | 1 |
Publications
Additional Notes
- Evaluated in canine melanoma skin samples using a 1:50 dilution and a donkey anti-rabbit secondary antibody (Thermo Fisher Scientific, A10043). Antibody should label mast cells in the skin and mast tumor cells. No signal observed but better control (mast cell tumor) is needed. Samples may have been overfixed (48 hours to 1 week+ in 10% formalin). Intense antigen retrieval (pH 6 and pH 9) was needed for well established antibodies (pan-cytokeratin, clone AE1/AE3).