UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
Q62230	Primary Antibody	CD169	Mouse	Rat	IgG2a	3D6.112	BioLegend	142404	PE	AB_10915697	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967
Q62230	Primary Antibody	CD169	Mouse	Rat	IgG2a	3D6.112	BioLegend	142404	PE	AB_10915697	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
Q62230	Primary Antibody	CD169	Mouse	Rat	IgG2a	3D6.112	BioLegend	142404	PE	AB_10915697	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Spleen	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
Q62230	Primary Antibody	CD169	Mouse	Rat	IgG2a	3D6.112	BioLegend	142404	PE	AB_10915697	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Thymus	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967

Publications

1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. Validated by Evelyn Kandov (No ORCID)

Mouse spleen: CD169 (green, catalog number 142404) and CD45 (blue, catalog number 103122)