| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A0A024FCJ9 | Primary Antibody | PD-1 | Canine | Goat | IgG | Polyclonal | R&D Systems | AF1086 | Unconjugated | AB_354588 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | 0000-0001-5088-7808 | NA | 0000-0001-5088-7808 | 1 |
Publications
Additional Notes
- Non-specific signal. Does not label Tfh cells in the germinal center (control) in the lymph node. Samples may have been overfixed (48 hours to 1 week+ in 10% formalin). Intense antigen retrieval (pH 6 and pH 9) was needed for well established antibodies (pan-cytokeratin, clone AE1/AE3).