| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NA | Secondary Antibody | Rabbit IgG (H+L) | Mouse | Goat | IgG | Polyclonal | Thermo Fisher Scientific | A-11037 | AF594 | AB_2534095 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Liver | NA | NA | NA | 1 mg/ml LiBH4 for more than 120 minutes | Yes | 0000-0003-4379-8967 [2, 4] | NA | 0000-0003-4379-8967 | 1 |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| NA | Secondary Antibody | Rabbit IgG (H+L) | Human | Donkey | IgG | Polyclonal | Thermo Fisher Scientific | A-21207 | AF594 | AB_141637 | Stock | Multiplexed 2D Imaging | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | NA | Yes | 0000-0003-4379-8967 [3] | NA | 0000-0003-4379-8967 |
Publications
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Q. Xu et al., “Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children”, Nat. Nat Immunol., , doi: 10.1038/s41590-022-01367-z.
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A. Gola et al., “Commensal-driven immune zonation of the liver promotes host defence”, Nature, 589(7840):131-136, 2021, doi:10.1038/s41586-020-2977-2.
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A. J. Radtke et al., “A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes”, bioRxiv, 2022, doi: 10.1101/2022.06.03.494716.
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A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Conjugate requires extended treatment with 1 mg/ml LiBH4 (120 minutes or more) to extinguish signal. Use in the last cycle or as a fiducial for IBEX experiments. For additional details on how to prepare samples (perfusion, no detergent) see [Gola et al. 2020].