UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q15306 Primary Antibody IRF4 Human Rabbit IgG Polyclonal Novus Biologicals NBP1-82814 Unconjugated AB_11038596 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
Q15306 Primary Antibody IRF4 Human Mouse IgG1 4G10 Novus Biologicals NBP1-47396 Unconjugated AB_10010349 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 2
Q15306 Primary Antibody IRF4 Human Mouse IgG1 MUM1p Novus Biologicals NB200-356 Unconjugated AB_10003502 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 3
Q15306 Primary Antibody IRF4 Human Sheep IgG Polyclonal R&D Systems AF5525 Unconjugated NA Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 4
Q15306 Primary Antibody IRF4 Human Mouse IgG1 MUM1p Novus Biologicals NB200-356 Unconjugated AB_10003502 Stock Cell DIVE-IBEX FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 5
Q15306 Primary Antibody IRF4 Human Mouse IgG1 MUM1p Novus Biologicals NB200-356 Unconjugated AB_10003502 Stock Cell DIVE-IBEX FFPE Lymph Node Healthy 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 [1, 2] NA 0000-0003-4379-8967 6

Publications

  1. A. J. Radtke et al., “Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients”, Cancer Cell, 42(3):444-463, 2024, doi: 10.1016/j.ccell.2024.02.001.

  2. A. J. Radtke, “OMAP-18: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Lymph Node with Cell DIVE-IBEX”, Human Reference Atlas, 2024, doi: 10.48539/HBM356.HGHM.686.

Additional Notes

  1. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody labels nuclei of plasma cells and plasmablasts.
  2. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody does not yield anticipated staining pattern.
  3. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody labels nuclei of plasma cells and plasmablasts but is dim. Amplification with secondary antibody is needed.
  4. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). High background and dim signal.
  5. Evaluated in human tonsil FFPE samples with CD20 (clone L26) and Hoechst (nuclear marker). Antibody labels nuclei of plasma cells and plasmablasts. Dual antigen retrieval with Leica Bond improves signal dramatically.
  6. IRF4 (Novus Biologicals NB200-356) is very dim. Use at a 1:10 dilution and amplify with a secondary antibody (Thermo Fisher Scientific A-21121).
Human normal LN: CD3 (blue, catalog number ab208514) and IRF4 (magenta, catalog number NB200-356 and A-21121)