| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Spleen | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Thymus | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 | 1 |
| P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100738 | BV421 | AB_11204079 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | 0000-0003-4379-8967 [1] | NA | 0000-0003-4379-8967 |
Publications
- A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117
Additional Notes
- Validated by Evelyn Kandov (No ORCID)
| Mouse spleen: CD4 (cyan, catalog number 100446), CD8 (magenta, catalog number 100738), and IgD (blue, catalog number 405730) |
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| Mouse thymus: CD8 (blue, catalog number 100738) and CD11c (magenta, catalog number 117312) |
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| Mouse gut: EpCAM (magenta, catalog number 118222), CD8 (blue, catalog number 100738), and CD31 (green, catalog number 102516) |
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| Mouse lymph node: CD8 (cyan, catalog number 100738), DEC205 (purple, catalog number 138204), and CD169 (yellow, catalog number 142404) |
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