UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
Q61830	Primary Antibody	CD206	Mouse	Rat	IgG2a	C068C2	BioLegend	141717	BV421	AB_2562232	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Thymus	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes + light	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967
Q61830	Primary Antibody	CD206	Mouse	Rat	IgG2a	C068C2	BioLegend	141717	BV421	AB_2562232	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lung	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes + light	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1
Q61830	Primary Antibody	CD206	Mouse	Rat	IgG2a	C068C2	BioLegend	141717	BV421	AB_2562232	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	1 mg/ml LiBH4 15 minutes + light	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967	1

Publications

1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

1. Validated by Evelyn Kandov (No ORCID)

Mouse thymus: CD206 (cyan, catalog number 141717) and Cytokeratin (purple, catalog number 628604)

Mouse lung: CD11c (magenta, catalog number 117312), CD206 (blue, catalog number 141717), and CD31 (green, catalog number 102520)

Mouse lymph node: CD206 (red, catalog number 141717) and CD106 (cyan, catalog number 105710)