UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Thymus NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lung NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1
Q61830 Primary Antibody CD206 Mouse Rat IgG2a C068C2 BioLegend 141717 BV421 AB_2562232 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1

Publications

  1. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Validated by Evelyn Kandov (No ORCID)
Mouse thymus: CD206 (cyan, catalog number 141717) and Cytokeratin (purple, catalog number 628604)
Mouse lung: CD11c (magenta, catalog number 117312), CD206 (blue, catalog number 141717), and CD31 (green, catalog number 102520)
Mouse lymph node: CD206 (red, catalog number 141717) and CD106 (cyan, catalog number 105710)