UniProt Accession Number	Reagent Type	Target Name / Protein Biomarker	Target Species	Host Organism	Isotype	Clonality	Vendor	Catalog Number	Conjugate	RRID	Availability	Method	Tissue Preservation	Target Tissue	Tissue State	Detergent	Antigen Retrieval Conditions	Dye Inactivation Conditions	Recommend	Agree	Disagree	Contributor	Notes
Q9Y5Y7	Primary Antibody	LYVE1	Human	Goat	IgG	Polyclonal	R&D Systems	AF2089	Unconjugated	AB_355144	Stock	IBEX2D Automated	1:4 Cytofix/Cytoperm Fixed Frozen	Jejunum	NA	0.3% Triton-X-100	NA	NA	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967
Q9Y5Y7	Primary Antibody	LYVE1	Human	Goat	IgG	Polyclonal	R&D Systems	AF2089	Unconjugated	AB_355144	Stock	IBEX2D Manual	1:4 Cytofix/Cytoperm Fixed Frozen	Liver	NA	0.3% Triton-X-100	NA	NA	Yes	0000-0003-4379-8967 [2, 1]	NA	0000-0003-4379-8967
Q9Y5Y7	Primary Antibody	LYVE1	Human	Goat	IgG	Polyclonal	R&D Systems	AF2089	Unconjugated	AB_355144	Stock	IBEX2D Automated	1:4 Cytofix/Cytoperm Fixed Frozen	Lymph Node	NA	0.3% Triton-X-100	NA	NA	Yes	0000-0003-4379-8967 [1]	NA	0000-0003-4379-8967
Q9Y5Y7	Primary Antibody	LYVE1	Human	Goat	IgG	Polyclonal	R&D Systems	AF2089	Unconjugated	AB_355144	Stock	Multiplexed 2D Imaging	FFPE	Lymph Node	Follicular Lymphoma	0.3% Triton-X-100	pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond	NA	Yes	0000-0003-4379-8967	NA	0000-0003-4379-8967	1

Publications

A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.
A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes