UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q02242 Primary Antibody PD-1 Mouse Rat IgG2a 29F.1.A12 BioLegend 135218 BV421 AB_2561447 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA 1 mg/ml LiBH4 15 minutes + light Yes 0000-0003-4379-8967 [2]; 0000-0003-1118-7432 NA 0000-0003-4379-8967  
Q15116 Primary Antibody PD-1 Human Mouse IgG1 EH12.2H7 BioLegend 329920 BV421 AB_10960742 Stock Multiplexed 2D Imaging 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967 1

Publications

  1. I. Sayin et al., “Spatial distribution and function of T follicular regulatory cells in human lymph nodes”, J Exp Med., 215(6):1531-1542, 2018, doi:10.1084/jem.20171940.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Clone EH12.2H7 labels antigen activated T cells in diverse tissues. Several conjugates are suitable for IBEX imaging. BV421 conjugate has been demonstrated in human lymph nodes and may be suitable for other tissues with low autofluorescence (see publication).
Mouse lymph node: PD-1 (green, catalog number 135218), CD23 (magenta, catalog number 101611), and IgD (blue, catalog number 405718)