UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 (Unconjugated) iF594 NA Custom IBEX2D Manual FFPE Liver Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256 1
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 (Unconjugated) iF594 NA Custom IBEX2D Manual FFPE Tonsil Metastatic Ovarian Cancer 0.3% Triton-X-100 pH 9.5 for 15 minutes in a pressure cooker (Borg Decloaker BD1000) 1 mg/ml LiBH4 15 minutes (3 exchanges for a total of 45 minutes) Yes 0000-0001-9561-4256 NA 0000-0001-9561-4256 1

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Additional Notes

  1. IBEX2D Manual is performed on tissue biopsies adhered to glass slides. After imaging, coverslips are soaked off and LiBH4 is placed directly onto the tissues. Three exchanges of LiBH4 (15 minute per incubation) are performed to ensure complete removal of signal. Conjugated with AAT Bioquest 1230.