UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 Unconjugated AB_355144 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Jejunum NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 Unconjugated AB_355144 Stock IBEX2D Manual 1:4 Cytofix/Cytoperm Fixed Frozen Liver NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [2, 1] NA 0000-0003-4379-8967  
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 Unconjugated AB_355144 Stock IBEX2D Automated 1:4 Cytofix/Cytoperm Fixed Frozen Lymph Node NA 0.3% Triton-X-100 NA NA Yes 0000-0003-4379-8967 [1] NA 0000-0003-4379-8967  
Q9Y5Y7 Primary Antibody LYVE-1 Human Goat IgG Polyclonal R&D Systems AF2089 Unconjugated AB_355144 Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA Yes 0000-0003-4379-8967 NA 0000-0003-4379-8967 1,2

Publications

  1. A. J. Radtke et al., “IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, Nat. Protoc., 17(2):378-401, 2022, doi: 10.1038/s41596-021-00644-9.

    A. J. Radtke et al., “Accompanying dataset for: IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues”, doi: 10.5281/zenodo.5244550.

  2. A. J. Radtke et al., “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, Proc Natl Acad Sci, 117(52):33455–33465, 2020, doi:10.1073/pnas.2018488117

Additional Notes

  1. Antibody labels lymphatic endothelial cells and macrophages as expected.
  2. Antibody labels lymphatic endothelial cells and vessels in FFPE tissues. Excellent antibody used throughout many studies. Used at a dilution of 1:100 with an anti-goat AF555 secondary antibody (Thermo Fisher Scientific catalog number A-21432).
Human follicular lymphoma FFPE: LYVE-1 (yellow, catalog number AF2089) and Hoechst (blue, catalog number 40046)