UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P15104 Primary Antibody Glutamine synthetase Human Rabbit IgG EPR13022(B) Abcam ab302584 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P15104 Primary Antibody Glutamine synthetase Human Mouse IgG2a OTI1F4 Novus Biologicals NBP2-70834AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P15104 Primary Antibody Glutamine synthetase Human Mouse IgG2bc GLUL/6600 Novus Biologicals NBP3-14023AF488 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1
P15104 Primary Antibody Glutamine synthetase Human Rabbit IgG EPR13022(B) Abcam Ab302584 AF488 NA Stock Multiplexed 2D Imaging FFPE Lymph Node Follicular Lymphoma 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 2

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Additional Notes

  1. Antibody did not label membrane or cytoplasm of tumor or stromal cells; however, a proper control was not available. The liver (positive control) FFPE sample had too much background in the AF488 channel.
  2. Antibody labeling is inconclusive and appears to label cytoplasm of most cells in a follicular lymphoma FFPE tissue section. Does colocalize with other glutamine synthetase antibody (NBP2-70834AF647). Used at a dilution of 1:50. Requires a good positive control and further testing.
Human follicular lymphoma FFPE: Glutamine synthetase (cyan, catalog number Ab302584)