UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
Q9BZS2 Primary Antibody FOXP3 Human Mouse IgG1 236A/E7 Thermo Fisher Scientific 17-4777-42 APC AB_10804651 Stock Multiplexed 2D Imaging FFPE Tonsil NA 0.3% Triton-X-100 pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond NA No 0000-0003-4379-8967 NA 0000-0003-4379-8967 1

Publications

Additional Notes

  1. Antibody does not label nuclei of Tregs. Amplification with an anti-APC AF647 (R&D Systems FAB8927R) secondary antibody is not sufficient.