UniProt Accession Number Reagent Type Target Name / Protein Biomarker Target Species Host Organism Isotype Clonality Vendor Catalog Number Conjugate RRID Availability Method Tissue Preservation Target Tissue Tissue State Detergent Antigen Retrieval Conditions Dye Inactivation Conditions Recommend Agree Disagree Contributor Notes
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Heart NA 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996 1
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Skin Infected 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996 2
P01732 Primary Antibody CD8 Human Mouse IgG1 C8/144B BioLegend 372906 AF647 AB_2650712 Stock IBEX2D Manual FFPE Cervix Cervical Cancer 0.3% Triton-X-100 pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) 1 mg/ml LiBH4 15 minutes Yes 0000-0002-7586-9996 NA 0000-0002-7586-9996 3

Publications

Additional Notes

  1. Evaluated in human FFPE tissues with CD45 and Hoechst (nuclear marker). Antibody yields anticipated staining pattern. Performed using manual IBEX protocol with antibody incubation using the Pelco Biowave microwave.
  2. Evaluated in human FFPE tissues from cutaneous leishmania lesion with CD3 and Hoechst (nuclear marker). Antibody yields anticipated staining pattern. Performed using manual IBEX protocol with antibody incubation using the Pelco Biowave microwave.
  3. Evaluated in human FFPE tissues with CD3 and Hoechst (nuclear marker). Antibody yields anticipated staining pattern. Performed using manual IBEX protocol with antibody incubation using the Pelco Biowave microwave.